Microbiology Lab Report

I. Title

Impact of Antibiotics on Bacterial Cultures

II. Abstract

This study investigates the effects of various antibiotics on the growth of bacterial cultures. Data were collected over a span of two weeks, and the results highlight the efficacy of different antibiotics in inhibiting bacterial growth.

III. Introduction

The purpose of this experiment is to evaluate the impact of specific antibiotics on bacterial colonies. Understanding how antibiotics affect bacterial growth can aid in developing better treatment protocols.

IV. Materials and Methods

Materials

Petri dishes
Agar medium
Microscope
Incubator
Antibiotic samples: Penicillin, Streptomycin, Tetracycline
Bacterial cultures: E. coli, S. aureus, P. aeruginosa

Methods

Prepare agar plates and inoculate with bacterial cultures.
Apply antibiotic discs to the agar plates.
Incubate the plates at 37°C for 24 hours.
Measure the zones of inhibition.
Record and analyze the data.

V. Results

Antibiotic	E. coli (mm)	S. aureus (mm)	P. aeruginosa (mm)
Penicillin	15	20	10
Streptomycin	25	30	15
Tetracycline	20	25	12

VI. Discussion

The results indicate that Streptomycin showed the highest efficacy against all three bacterial cultures, as evident from the largest zones of inhibition. Penicillin and Tetracycline also inhibited bacterial growth but to a lesser extent.

VII. Conclusion

Streptomycin was the most effective antibiotic against the tested bacterial strains. Further research should explore the mechanisms of antibiotic resistance and test a broader range of antibiotics.

VIII. Acknowledgments

This report is prepared by

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